plasmid 10828 25 Search Results


plasmid 10828 25  (Addgene inc)
92
Addgene inc plasmid 10828 25
Plasmid 10828 25, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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source strains escherichia coli dh10b escherichia coli s17 1 λpir donor strain methylomicrobium alcaliphilum 20z  (DSMZ)
96
DSMZ source strains escherichia coli dh10b escherichia coli s17 1 λpir donor strain methylomicrobium alcaliphilum 20z
Source Strains Escherichia Coli Dh10b Escherichia Coli S17 1 λpir Donor Strain Methylomicrobium Alcaliphilum 20z, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+10828+25/Escherichia+coli/ppr0369890-120-14-32
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source strains escherichia coli dh10b escherichia coli s17 1 λpir donor strain methylomicrobium alcaliphilum 20z - by Bioz Stars, 2026-10
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hindiii  (New England Biolabs)
99
New England Biolabs hindiii
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pxl002  (Addgene inc)
92
Addgene inc pxl002
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pspax2  (Addgene inc)
98
Addgene inc pspax2
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165 pxl004  (Addgene inc)
92
Addgene inc 165 pxl004
165 Pxl004, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmd2 g  (Addgene inc)
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Addgene inc pmd2 g
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https://www.bioz.com/product/plasmid+10828+25/pMD2%2EG+(Plasmid+%2312259)/10__1038_slash_nprot__2012__150-82-242-243
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es 006 b vector red alkaline phosphatase  (Vector Laboratories)
96
Vector Laboratories es 006 b vector red alkaline phosphatase
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chir99021  (Selleck Chemicals)
96
Selleck Chemicals chir99021
Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM <t>CHIR99021</t> treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.
Chir99021, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fugene hd transfection reagent  (Promega)
90
Promega fugene hd transfection reagent
Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM <t>CHIR99021</t> treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.
Fugene Hd Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtesr1  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mtesr1
Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM <t>CHIR99021</t> treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.
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matrigel  (Becton Dickinson)
90
Becton Dickinson matrigel
Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM <t>CHIR99021</t> treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.
Matrigel, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM CHIR99021 treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.

Journal: Nature protocols

Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions.

doi: 10.1038/nprot.2012.150

Figure Lengend Snippet: Figure 5 | Quantitative analysis of cardiomyocytes differentiated from hPSCs via small-molecule modulation of Wnt signaling. (a,b) Cardiomyocytes were generated from 19-9-11 iPSCs using the protocol described in Figure 3, with 12 µM CHIR99021 treatment at day 0 and 5 µM IWP2 treatment at day 3. At day 20, cells were analyzed for cTnT/SMA and cTnT/MLC2a expression (a) and MF20/Ki67 and MF20/BrdU incorporation by flow cytometry (b). SMA, smooth muscle actin.

Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) psPAX2 (Addgene, cat. no. 12260) (Step 12A only) pMD2.G (Addgene, cat. no. 12259) (Step 12A only) pXL002 (Addgene, cat. no. 36297) (Step 12A only) • • • • • • • • • • • • • • • • • • • • • • • © 20 12 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . nature protocols | VOL.8 NO.1 | 2013 | 165 pXL004 (Addgene, cat. no. 36311) (Step 12A only) Puromycin (10 mg ml − 1; Life Technologies, cat. no. A11138-03) (Step 12A only) Qiagen EndoFree plasmid maxi kit (Qiagen, cat. no. 12362) (Step 12A only) QuantiTect SYBR Green PCR kit (Qiagen, cat. no. 204141) (Step 12A only) RNeasy mini kit (Qiagen, cat. no. 74104) (Step 12A only) Sodium pyruvate (Life Technologies, cat. no. 11360-070) (Step 12A only) PBS (Sigma, cat. no. D8537) Triton X-100 (Sigma, cat. no. T8532) Versene (Life Technologies, cat. no. 15040-066) Y27632 (Tocris, cat. no. 1254) Liquid nitrogen Ethanol, 70% (vol/vol) Milli-Q water Sodium tetraborate (Sigma, cat. no. 221732) Primers for quantitative RT-PCR (Step 12A only).

Techniques: Generated, Expressing, BrdU Incorporation Assay, Flow Cytometry